FAQ: What temperature is Thermostable USER® III Enzyme active?

Thermostable USER III Enzyme is active at temperatures >50°C, with optimal activity observed at 65°C. Minimal activity is observed at temperatures less than 50°C. Like USER Enzyme (NEB #M5505), Thermostable USER III Enzyme cannot be heat inactivated and must be removed by other methods (e.g., column purification/phenol chloroform extraction).