Thermostable USER III Enzyme is 100% active in Thermopol Buffer and has limited activity in other buffers. Activity in NEBuffers at 65°C is as follows:
Additionally, Thermolabile USER II Enzyme is 100% active in many polymerase and PCR buffers (see chart below).
|Buffer||Activity (Relative to Thermopol Buffer)|
|Thermopol II Reaction Buffer (NEB #B9004)||100%|
|Q5® Reaction Buffer (NEB #B9027)||50%|
|Colorless GoTaq® Flexi Buffer (Promega®)||75%|
|PCR Buffer (Qiagen®)||75%|
|AmpliTaq Gold® 360 Buffer (ThermoFisher®)||100%|
|Fast Start™ Taq DNA Pol PCR Buffer (Sigma Aldrich)||0%|
|Ex Taq Buffer (Takara)||0%|
|Dream Taq Buffer (ThermoFisher®)||75%|
|Platinum II PCR Buffer (Invitrogen)||75%|
- AmpliTaq Gold® and Thermo Fisher® are registered trademarks and property of Thermo Fisher Scientific.
- GoTaq® and Promega® are registered trademarks of Promega, Inc.
- Qiagen® is a registered trademark of Qiagen, Inc.
- Fast Start™ is a trademark of Sigma Aldrich.