FAQ: Are modified nucleotides included in the kit?

Modified nucleotides are not included in the kit but can be purchased separately. NEB can provide N1-Methyl-Pseudouridine-5’-Triphosphate (NEB #N0431), 5-Methyl-Cytidine-5’-Triphosphate (NEB #N0432), Pseudouridine-5’-Triphosphate (NEB #N0433) and 5-Methoxy-Uridine-5’-Triphosphate (NEB #N0434). The kit manual includes a detailed protocol for using modified nucleotides in the transcription reaction.