HomeFAQsCan modified nucleotides be used with the HiScribe® T7 mRNA Kit with CleanCap® Reagent AG?
FAQ: Can modified nucleotides be used with the HiScribe® T7 mRNA Kit with CleanCap® Reagent AG?
Yes. For complete substitution, leave out the unlabeled NTP provided with the kit and supplement with the same final concentration of a modified NTP (CTP or UTP) of your choice. Modified ATP should be avoided as it will interfere with CleanCap® Reagent AG incorporation. To note, some modified NTPs may impact final yield of the synthesis reaction and ratios of modified:unmodified NTPs may need to be optimized for partial substitutions. Please refer to the protocol in the product manual for use with modified NTPs.