FAQ: Can the Monarch HMW DNA Extraction Kits be used to isolate BAC DNA?

We have not tested this, but expect this should work as the BAC constructs are in the optimal size range for bead binding. However, BACs usually are very low copy number constructs, and a significant amount of culture will be needed to accumulate enough BAC DNA for the bead binding (> 5 μg). This will lead to an increase in the buffer volumes needed in the alkaline lysis. Altogether, the volume of the 3 alkaline lysis buffers should not surpass 1100 μl so that the DNA can still be bound to the beads in a 2 ml reaction tube. Some optimization work may be required, but it should be possible to connect the alkaline lysis chemistry with the bead binding and purification part of the Monarch protocols.