FAQ: In the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, what is the target of the Internal Control Primer Mix (rActin)?

The Internal Control LAMP Primer Mix targets actin RNA at an exon-exon junction. The primers also include a region that matches an ankyrin sequence in gDNA so low levels of amplification can be observed from mammalian gDNA but this is a minor contribution to overall signal given that rActin RNA is typically present at much higher copy numbers than gDNA.