FAQ: I currently use mMessage mMachine® T7 Ultra Transcription Kit, which mRNA synthesis kit from NEB should I use?

We recommend the HiScribe T7 ARCA mRNA kit (with tailing)(E2060) which can be used to co-transcriptionally cap with ARCA and subsequently add a poly(A) tail using E. coli Poly(A) Polymerase. The HiScribe kit contains reagents and protocols for complete co-transcriptional ARCA capping and tailing of synthesis reactions.