FAQ: Can Poly(U) Polymerase incorporate GTPs onto an RNA that contains chemically modified or 2-O-methyl groups on its 3’ end?

Yes. However, Poly(U) Polymerase has a strong primer bias based upon the last few nucleotides on the 3’ end so in a mixed RNA population where some RNA may have modifications such as being double stranded or chemically modified with 2-O-methyl groups a longer incubation of the reaction may be required.