HomeFAQsWill the ligation reaction using T4 RNA Ligase 1 produce circles or duplex products?
FAQ: Will the ligation reaction using T4 RNA Ligase 1 produce circles or duplex products?
The reaction can produce both. The unit is defined by producing circles using rA-20mer as a substrate. Increasing the concentration, length and adding PEG can increase the number of intermolecular ligations. Blocking one end of the molecule with a dideoxy terminator will prevent the molecule from forming a circle.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.