Home FAQs Why are there no colonies or no growth in liquid culture (C3037)?

FAQ: Why are there no colonies or no growth in liquid culture (C3037)?

There may be basal expression in the NEB Express host. If toxicity of the expressed protein is likely,
» Incubation at 30°C or room temperature may alleviate toxicity issues.
» In addition, check antibiotic concentration (test with control plasmid).