Home FAQs What is the optimal heat shock time for this strain (NEB #C3037H and NEB #C3037I)?

FAQ: What is the optimal heat shock time for this strain (NEB #C3037H and NEB #C3037I)?

Heat shock at 42ºC for 20 seconds results in the highest transformation efficiency for NEB Express Iq competent E.coli (NEB #C3037H and NEB #C3037I). Expect approximately 50% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).

Videos

  1. TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.