FAQ: What is LysY?

LysY is a variant of T7 lysozyme lacking amidase activity making the cells less susceptible to lysis during induction, while retaining the ability to inhibit T7 RNA Polymerase. Basal expression of the target gene is minimized without inhibiting IPTG-induced expression. LysY is encoded on a single-copy miniF plasmid that does not require antibiotic selection for propagation.