HomeFAQsWe have been using your Endoproteinase GluC as a Histidine-tagged protein marker for western blots. The molecular weight listed on your website for this product is 29849 Daltons. Does that include the 6-his added as the tag?
FAQ: We have been using your Endoproteinase GluC as a Histidine-tagged protein marker for western blots. The molecular weight listed on your website for this product is 29849 Daltons. Does that include the 6-his added as the tag?
We have also used it as a His-tagged marker protein. The 29849 Daltons mass does include the 6-His tag but you should be aware that not every Endoproteinase GluC contains all six His residues. As a part of the maturation (conversion of the zymogen to the active protease) the C- terminal of the protein containing the His-tag also gets nibbled producing a distribution of lengths of the remaining tag. Some 6-His remains, but we believe the predominate species is 4 His residues with a distribution from 1-6.
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