HomeFAQsNEB's data card for hNEIL1 states that this enzyme does not cleave 5-hmU containing substrates. However, there are two publications by the Qiu-Mei Zhang group from Kyoto University demonstrating cleavage of 5-hmU oligos by both hNEIL1 and Nei, the E.coli homolog. Can you perhaps shed some light on this matter?
FAQ: NEB's data card for hNEIL1 states that this enzyme does not cleave 5-hmU containing substrates. However, there are two publications by the Qiu-Mei Zhang group from Kyoto University demonstrating cleavage of 5-hmU oligos by both hNEIL1 and Nei, the E.coli homolog. Can you perhaps shed some light on this matter?
Our data card is correct. hNEIL1 activity was tested on 34-mer oligonucleotide duplexes containing 5-hmU opposite to A or G. There was no activity observed when 1.25-40 units of hNEIL1 were used.
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