FAQ: How should the Red Loading Buffer be used?

1.Add 1/10 volume of the 30X DTT solution to 1 volume 3X Red Loading Buffer.
2.Add 1/2 volume of the above mix to the protein sample.
3.Heat at 100°C for 5 minutes to denature the protein.
4.Spin for 30 seconds in a microfuge to remove precipitated material.
5.Load onto gel.