FAQ: How do I know if the glucosylation of 5-hmC in my DNA is complete?

One unit of T4-BGT glucosylates 0.5 μg of T4 gt DNA to completion. Our experiments show that 10 units of T4-BGT is more than enough to glucosylate 1.5 μg of genomic DNA overnight as indicated in protocol (see manual page 6).