Home FAQs Does Streptomycin and/or Spectinomycin need to be added when growing SHuffle strains?

FAQ: Does Streptomycin and/or Spectinomycin need to be added when growing SHuffle strains?

No, resistance to these antibiotics is due to gene(s) stably integrated into the chromosome.
Expression plasmids with Streptomycin or Spectinomycin resistance markers should be avoided.