FAQ: Can I use assay kits designed for other reporters (Gaussia, Renilla & Firefly luciferases) to assay CLuc activity?

No. Cypridina Luciferase catalyzes the light reaction using a different substrate that is not the same as those for Gaussia, Renilla & Firefly luciferases. Therefore, the CLuc activity can only be assayed by using the BioLux CLuc Assay Kit (NEB #E3309).