
Applications of IPL
Return to Peptide LigationSince IPL allows the fusion of synthetic peptides, as well as bacterially expressed proteins, with an N-terminal cysteine, to a protein expressed in the IMPACT system, IPL has been used in a variety of ways including:
- The expression of cytotoxic proteins (1).
- The labeling of proteins with radioactive compounds as well as with synthetic peptides containing biotin or fluorescein (1-3).
- The study of protein-protein interactions (4).
- The generation of kinase substrates by varying the kinase recognition site at the protein level instead of at the DNA level (5, 6).
- The generation of phosphatase substrates (7).
- The isotopic labeling of proteins for NMR analysis (8).
- Generation of substrates for protein arrays (9).
- Site specifically incorporating lipid moieties into a protein (10).
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