Quick Tips - How much should I dilute my adapter?

This video describes the factors that need to be taken into consideration when calculating your adapter dilution.


I often get asked “How much I should dilute my adaptor?”

We always recommend that you check the NEB manual for adaptor dilution. However, the ideal adaptor dilution may be dependent on your input type and the quality of that input. If you find after your final cleanup that you have an adaptor dimer, you may need to consider further diluting your adaptor.

Remember, if you have any other questions, please contact us at info@neb.com.

Related Videos

  • NEBNextVideo_12Tips_thumb
    12 Quick Tips for NGS Library Preparation
  • Optimization_of_NGS_TN
    Optimization of NGS Library Preparation: Low Inputs and Fast, Streamlined Workflows
  • Library Preperation For NGS
    Library prep for NGS
Visit NEB’s Video Library
Loading Spinner