Protein Kinase Substrate Recognition
NEB offers protein kinases for a number of different specificity motifs. Phosphoacceptor residue is indicated in red, amino acids which can function interchangeably at a particular residue are separated by a slash (/), and residues which do not appear to contribute strongly to recognition are indicated by an "X".
| Protein Kinase | NEB # | Recognition Determinant | Size |
|---|---|---|---|
| cAMP-dependent Protein Kinase (PKA), Catalytic Subunit | P6000S/L | R-R-X-S/TYY= hydrophobic residue | 100,000/500,000 units |
| Casein Kinase II (CK2) | P6010S/L | S-X-X-E/D | 10,000/50,000 units |
Single-letter Amino Acid Code:
A = alanine, C = cysteine, D = aspartic acid, E = glutamic acid, F = phenylalanine, G = glycine, H = histidine, I = isoleucine, K = lysine, L = leucine, M = methionine, N = asparagine, P = proline, Q = glutamine, R = arginine, S = serine, T = threonine, W = tryptophan, V = valine, Y = tyrosine, X = any amino acid
Note: More specific information on recognition determinants for each kinase can be found on the corresponding product page.
