Enzymes For Innovation

The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes. However, in molecular biology, new tools can often lead to new discoveries. Taking advantage of the enzymology expertise at NEB, we are offering novel enzymes with interesting and unique activities for manipulating DNA, RNA, proteins and glycans, even if specific applications for them have yet to be discovered. Our hope is that by engaging researchers' imaginations, our “Enzymes for Innovation” initiative will enable the development of new molecular techniques that so often lead to new discoveries.

Products Available

5-hydroxymethyluridine DNA Kinase M0659 M0659-specificity

5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA.
Boletopsis grisea Lectin (BGL)



Enables enrichment of GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows.

EcoGII Methyltransferase M0603 EFI_EcoGII_Methyltransferase

EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
Endoglycoceramidase I (EGCase I) P0773 EFI_EGCaseI

Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids.
Immobilized T4 DNA Ligase


Immobilized T4 DNA Ligase is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, the enzyme can be removed using a magnet and can be re-used. 

Msz Exonuclease I (MszExol)
M0527 M0527
Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C.
Nb.BssSI R0681 EFI_NbBssSI

Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1).
NudC Pyrophosphatase M0607


NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding).

RtcB Ligase M0458 EFI_RtcBligase

Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl. 
Sce PUS1


Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer.

TelN Protelomerase M0651  EFI_TelN_Protelomerase

Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
Thermostable FEN1 M0645  EFI_ThermostableFEN1

Catalyzes the cleavage of 5´ DNA flaps from branched double-stranded DNA substrates.
Tte UvrD Helicase M1202

Tte UvrD Helicase

Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.

 Tth Argonaute  M0665


TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide.

If you are looking for an enzyme functionality that is not currently available, contact EnzymesForInnovation@neb.com.

Have you found a good application for one of the enzymes listed above? we would love to hear about it!


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Q: What are Enzymes for Innovation?
A: Enzymes for Innovation (EFI) is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities that are not commercially available elsewhere at the quality that you would expect from NEB.  If you have an idea for an “Enzyme for Innovation” with a suggested application that may be useful, please email us at EnzymesForInnovation@neb.com. For more information, please view video.

Q: Can I submit an Enzyme for Innovation idea?
A: Yes! Please email EnzymesForInnovation@neb.com with ideas for new enzymes (please note enzymes should modify either DNA, RNA, or protein, or be useful in glycobiology applications).

Q: Can I purchase large amounts of an existing Enzyme for Innovation?
A: Yes, if you have use for large amounts of an Enzyme for Innovation, please contact NEB at EnzymesForInnovation@neb.com. Please note that there may be limited availability and thus extended lead times required for large orders. Additional QC testing may also be necessary to meet your specific application and needs.

Q: What quality controls are performed to validate Enzymes for Innovation?
A: Products designated as Enzymes for Innovation pass stringent quality controls, including removal of contaminants and characterization of enzyme stability.
Dance, A. (2021) Refining the toolkit for sugar analysis. Nature, 599, 168-169. DOI: https://doi.org/10.1038/d41586-021-03000-9


  • WhatAreEnzymesForInnovation

    What are Enzymes for Innovation?

    NEB’s Chief Scientific Officer, Sir Richard Roberts, describes the Enzymes for Innovation initiative, and the importance of new enzyme discovery and screening.