Adenosine deamination in RNA

We are applying a ReCappable-seq approach to reveal the modification status of position 1 Adenosine of capped RNA in mammalian cells.
We are also exploring adenosine deaminases with the goal of identifying modified adenosines in RNA at single nucleotide resolution. We have observed positional effects upon in vitro deamination of adenosine residues in RNA which we are interpreting as structural effects on the accessibility of adenosines to non-ADAR adenosine deaminases.


Overlay of three adenosine deaminase ligand binding sites.