Home Protocols Where can I find protocols for using the NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330)?

Where can I find protocols for using the NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330)?

Protocols for using NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330) reagents can be found within the product manual, including:

  • Section 1: Protocol for use with NEBNext® Poly(A) mRNA Magnetic Isolation Module
  • Section 2: Protocol for use with NEBNext rRNA Depletion Kits
  • Appendix A: Recommendations for Customized Adaptor Dilutions and Cycling per Input
  • Appendix B: Recommendations for Use with Indexed UMI Adaptors
  • Appendix C: Recommendations for Use with FFPE RNA
  • Appendix D: Recommendations for Fragmentation Time for Longer RNA Inserts