Where can I find protocols for using the NEBNext UltraExpress™ RNA Library Prep Kit (NEB #E3330)?
Protocols for using NEBNext UltraExpress™ RNA Library Prep Kit (NEB #E3330) reagents can be found within the product manual, including:
- Section 1: Protocol for use with NEBNext® Poly(A) mRNA Magnetic Isolation Module
- Section 2: Protocol for use with NEBNext rRNA Depletion Kits
- Appendix A: Recommendations for Customized Adaptor Dilutions and Cycling per Input
- Appendix B: Recommendations for Use with Indexed UMI Adaptors
- Appendix C: Recommendations for Use with FFPE RNA
- Appendix D: Recommendations for Fragmentation Time for Longer RNA Inserts
