Home Protocols Where can I find protocols for using the NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330)?

Where can I find protocols for using the NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330)?

Protocols for using NEBNext UltraExpress® RNA Library Prep Kit (NEB #E3330) reagents can be found within the product manual, including:

  • Section 1A: Express protocol for use with: NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), NEBNext UltraExpress RNA Library Prep Kit (NEB #E3330), NEBNext Adaptor for Illumina (non-indexed)
  • Section 1B: Standard protocol for use with: NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), NEBNext UltraExpress RNA Library Prep Kit (NEB #E3330), NEBNext Adaptor for Illumina (non-indexed)
  • Section 2: Protocol for use with: NEBNext rRNA Depletion Kits, NEBNext UltraExpress RNA Library Prep Kit (NEB #E3330), NEBNext Adaptor for Illumina (non-indexed)
  • Appendix A: Recommendations for Customized Adaptor Dilutions and Cycling per Input
  • Appendix B: Recommendations for Use with Unique Dual Indexed UMI Adaptors for Illumina
  • Appendix C: Recommendations for Use with FFPE RNA
  • Appendix D: Recommendations for Fragmentation Times for Longer RNA Inserts

Please also see protocol: Where can I find protocols for using NEBNext® RNA Library Prep reagents?
For more information on protocols using various NEBNext RNA Library Prep Kit reagents.