Where can I find protocols for using the NEBNext UltraExpress® DNA Library Prep Kit (NEB #E3325)?

Protocols for using NEBNext UltraExpress® DNA Library Prep Kit (NEB #E3325) reagents can be found within the product manual, including:

  • Protocols for End Prep, Adaptor Ligation, PCR Enrichment, and Phased Bead Cleanup
  • Appendix A: Recommendations for Customized Adaptor Dilutions and Cycling per Input