Protocol for cleavage with Thermostable OGG (NEB #M0464)

Note: Thermostable OGG will function at temperatures between 25-80°C, but functions optimally between 65-75°C.

Set up the following reaction:

Components 20 µl reaction Final conc.
Thermostable OGG 1 µl  


8-oxoG-containing DNA Up to 80 pmol  


rCutSmart Buffer (10X) 2 µl 1X
H20 up to 20 µl   

Gently mix the reaction by pipetting up and down and microfuge briefly.

Incubate at 65-75°C for 1 hour.