Protocol for conversion of Uridine to Pseudouridine using Sce PUS1 (NEB #M0526)

  1. Prepare 100 µl reaction as described below:

    100 µl Reaction 
    up to 1 µg
    NEBuffer r1.1 (10X)
    10 µl
    Sce PUS1
    1 µl (100 pmoles)
    Nuclease-free water
    to 100 µl

  2. Incubate at 30°C for 2 hours

  3. The reaction may be stopped by the addition of 1 µl of NEB Proteinase K and 15 min incubation at 37°C. Subsequent RNA purification using the appropriate NEB Monarch RNA Cleanup Kit can be used to clean up the RNA for downstream applications.


Links to this resource

Related Products:
Sce PUS1