Protocol for decapping of total RNA (NEB #M0463)

Component Concentration Final Concentration Volume
Total RNA   1-5 µg/reaction  
yDcpS 200 units/µl 4 units/µl 1µl
yDcpS Reaction Buffer 10X 1X 5 µl
Nuclease Free Water     Up to 50 µl
    Final Volume 50 µl

Incubate at 37˚C for 1 hour.

Inactivate yDcpS by incubation at 70˚C for 15 minutes.

Proceed with RNA analysis.

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