Protocol for T4 PDG (M0308)

1) Reaction set-up:

DNA 10 pmol
T4 PDG Reaction Buffer (10X) 2-5 µl
T4 PDG (T4 Endonuclease V)  1 µl (10 units)
Recombinant Albumin 0.5 - 2.5 µl
H2O Up to 20-50 µl

2) Incubate at 37°C for 30 minutes.

Addition of SDS to a final concentration of 0.5%, or 1µl phenol, in 6X Loading dye to the sample before running the gel will improve electrophoresis by dissociating the protein from the DNA.