Protocol for Endonuclease IV (M0304)
1) Reaction set-up:
| DNA | 10 pmol |
| NEBuffer 3 (NEB #B7003) (10X) | 1-5 µl |
| Endonuclease IV | 1 µl (10 units) |
| H2O | Up to 10-50 µl |
2) Incubate at 37°C for 1 hour.
1) Reaction set-up:
| DNA | 10 pmol |
| NEBuffer 3 (NEB #B7003) (10X) | 1-5 µl |
| Endonuclease IV | 1 µl (10 units) |
| H2O | Up to 10-50 µl |
2) Incubate at 37°C for 1 hour.
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