Home Protocols Endo-α-N-Acetyl Galactosaminidase (P0733)

Endo-α-N-Acetyl Galactosaminidase (P0733)

Overview

Reaction Conditions:

Introduction

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:

Protocol

  1. Combine 10-20 µg of glycoprotein, 1 µl of 10X Glycoprotein Denaturing Buffer and H2O (if necessary) to make a 10 µl total reaction volume.
  2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.
  3. Make a total reaction volume of 20 µl by adding 2 µl 10X G7 Reaction Buffer, 2 µl 10% NP40, 2 µl Neuraminidase, H2O and 1-5 µl Endo-α-N-Acetylgalactosaminidase.
  4. Incubate reaction at 37°C for 1-4 hours.