Adaptor Ligation of dA-Tailed DNA (E6040)


  1. Mix the following components in a sterile microfuge tube:

    dA-Tailed DNA:   25 μl
    Quick Ligation Reaction Buffer (5X):   10 μl
    15 μM DNA Adaptors*:   10 μl
    Quick T4 DNA Ligase:   5 μl
    Total volume:   50 μl

    * Adaptors are not provided; please use adaptors appropriate to specific application. If necessary adjust the adaptor concentration to a final adaptor to DNA molar ratio of 10:1.
  2. Incubate in a thermal cycler for 15 minutes at 20°C.
  3. Purify DNA sample on one column and elute in 30 μl of sterile dH2O or elution buffer.