Adaptor Ligation of dA-Tailed DNA (E6040)

Protocol

1. Mix the following components in a sterile microfuge tube:
   
   dA-Tailed DNA:   25 μl
   Quick Ligation Reaction Buffer (5X):   10 μl
   15 μM DNA Adaptors*:   10 μl
   Quick T4 DNA Ligase:   5 μl
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   Total volume:   50 μl
   
   * Adaptors are not provided; please use adaptors appropriate to specific application. If necessary adjust the adaptor concentration to a final adaptor to DNA molar ratio of 10:1.
2. Incubate in a thermal cycler for 15 minutes at 20°C.
3. Purify DNA sample on one column and elute in 30 μl of sterile dH₂O or elution buffer.