NEBNext Quick Ligation Module Protocol (E6040)


  1. Mix the following components in a sterile microfuge tube:

    End Repaired, Blunt or dA-Tailed DNA:   25 μl
    Quick Ligation Reaction Buffer (5X):   10 μl
    10 µM DNA Adaptors (not provided; please use adaptors appropriate to specific application):   10 μl
    Quick T4 DNA Ligase:   5 μl
    Total volume:   50 μl
  2. Incubate in a thermal cycler for 15 minutes at 20°C.
  3. Purify DNA sample on one column and elute in 30 μl of sterile dH2O or elution buffer.