Home Protocols Carrier Protein 39 (Western) Protocol 2: Analysis of Ligation by Coomassie Blue Stained SDS-PAGE

Carrier Protein 39 (Western) Protocol 2: Analysis of Ligation by Coomassie Blue Stained SDS-PAGE

Protocol

  1. Dissolve your peptide in water to a concentration of 1 mM.
  2. Thaw the carrier protein at room temperature.
  3. Mix the following (in order):
  4. Incubate at room temperature for 15-60.
  5. Add 12.5 µl of 3X SDS Sample Buffer.
  6. Heat at 95°C for 5 minutes.
  7. Load 15 µl/lane (~ 0.8 µg carrier protein) for Coomassie Blue staining.