Second Strand cDNA Synthesis (E6114)


  1. Add 48 μl Nuclease-Free Water to the First Strand Synthesis reaction.
  2. To the reaction then add:
    10X Second Strand Synthesis Reaction Buffer: 8 μl
    Second Strand Synthesis Enzyme Mix: 4 μl
    Total volume: 80 μl
  3. Mix thoroughly by gentle pipetting.
  4. Incubate in a thermal cycler for 2.5 hours at 16°C.