NEBNext End Repair Module Protocol (E6260)


Starting Material: 10 ng of chromatin-immunoprecipitated (ChIP) qPCR verified or control DNA in ≤ 85 μl of water or elution buffer.


  1. Mix the following components in a sterile microfuge tube:
    ChIP DNA 1–85 μl
    NEBNext End Repair Reaction Buffer (10X) 10 μl
    NEBNext End Repair Enzyme Mix 5 μl
    Sterile H2O variable
    Total volume 100 μl
  2. Incubate for 30 minutes at room temperature.
  3. Purify the end repaired DNA using Agencourt AMPure XP beads.