NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)


  1. After completion of the End Repair/dA-Tailing program add 1.0 μl of adaptor (or vector if generating an expression library), to the reaction tube. (Adaptors/vectors are not provided. Please use adaptors/vectors appropriate to specific application).

  2. Add 1.0 μl of Quick T4 DNA Ligase. Vortex briefly to mix, followed by a quick spin.

  3. Incubate for 10 minutes at 25°C.