Adaptor or Vector Ligation of cDNA Library (E6114)


  1. Mix the following components in a sterile microfuge tube: 

    End Repaired, Blunt DNA:   variable
    NEBNext Quick Ligation Reaction Buffer (5X):   40 μl
    DNA Adaptors or Vector variable T4 DNA Ligase:   10 μl
    Nuclease-Free Water:   variable 
    total volume:   200 μl
  2. Incubate in a thermal cycler for 15 minutes at 20°C.
  3. Purify DNA sample on one column.