Adaptor Ligation of End Repaired DNA (E6260)


  1. Mix the following components in a sterile microfuge tube:
    End Repaired, Blunt DNA 30 μl
    NEBNext Quick Ligation Reaction Buffer (5X) 20 μl
    *P1 Adaptor (2.5 pmol / μl) 1 μl
    *P2 Adaptor (2.5 pmol / μl) 1 μl
    Quick T4 DNA Ligase 1 μl
    Sterile H2O 47 μl
    Total volume 100 μl
    *Adaptors are not included, use adaptors appropriate to specific application.
  2. Incubate at room temperature for 10 minutes.