FAQ: How do I rehydrate the LyoPrime LAMP mix? Can it be resuspended at a higher concentration?

Vial format: To rehydrate the lyophilized mix as a 2X Master Mix, remove the vial cap and stopper, add 675 μl nuclease-free water, and briefly mix by pipetting or gentle vortexing. The lyophilized mix can be rehydrated at up to 4X to accommodate higher sample volumes. However, reconstitution at higher concentrations than 2X may negatively impact time to detection for some targets. Please also see the protocol for additional information.

Plate format: The lyophilized mix in each well can be rehydrated directly with primers and template to achieve 1X Master Mix (25 μl reaction volume). Therefore, it is highly recommended to prepare an assay mix including primers that is dispense into each well. Following adding assay mix, briefly mix by pipetting or vortexing using microplate shaker and then add template. Rehydration information can be found on the protocol page here and is also demonstrated in the product overview video.
  1. Image of LyoPrime WarmStart Fluorescent LAMP Mix in 96 well plate

    LyoPrime WarmStart™ Fluorescent LAMP RT-LAMP Mix (with UDG)

    Learn about the flexibility of LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) and find recommendations for how to use and store this product.