FAQ: How much RNase 4 (NEB #M1284) should I use for DNA-probe directed 5´-cap mRNA analysis?

The current recommendation is to dilute RNase 4 (50,000 U/ml) 1:30 in 1X NEBuffer r1.1. Adding 1 µl of diluted RNase 4 will be sufficient in reactions that contain 5 µg target RNA hybridized with 40 pmol of DNA probe (2023 Wolf et al. ACS Pharmacol. Transl. Sci.).

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