Home FAQs What is the difference between EnGen SpRY Cas9, EnGen Spy Cas9 HF1 (NEB #M0667) and EnGen Spy Cas9 NLS (NEB #M0646)?

FAQ: What is the difference between EnGen SpRY Cas9, EnGen Spy Cas9 HF1 (NEB #M0667) and EnGen Spy Cas9 NLS (NEB #M0646)?

EnGen Spy Cas9 NLS (NEB #M0646) encodes the wild-type Cas9 amino acid sequence whereas EnGen Spy Cas9 HF1 (NEB #M0667) encodes four point mutations in the Cas9 amino acid sequence (N497A, R661A, Q695A, Q926A) for reduction of off-target cleavage. EnGen SpRY Cas9 encodes 11 point mutations in the Cas9 amino acid sequence (A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N1317R, A1322R, R1333P, R1335Q, T1337R) for a relaxed PAM requirement.