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FAQ: Is Duplex DNase inhibited by salt?

Duplex DNase is inhibited by increasing amounts of salt. We recommend keeping salt concentrations below 50 mM (final) in the Duplex DNase reaction.

Duplex DNase Activity is inhibited by increasing salt concentration

M7635 Salt Concentration

Duplex DNase activity was measured by monitoring the increase in fluorescence when a quenched 35 nt hairpin dsDNA substrate is cleaved in 1X Reaction Buffer (10 mM Tris-HCl, pH 7.6, 2.5 mM MgCl2, 0.5 mM CaCl2) with increasing salt concentration, as indicated.