Home FAQs Why, at some stages of the E5hmC-seq™ Conversion Module protocol, do the NEBNext® Sample Purification Beads behave differently when cleaning up the sample?

FAQ: Why, at some stages of the E5hmC-seq™ Conversion Module protocol, do the NEBNext® Sample Purification Beads behave differently when cleaning up the sample?

The behavior of the beads does change depending on the clean-up and the previous E5hmC-seq Conversion Module reaction step. The bead pellet dries quickly after the glucosylation reaction, so it is important to work quickly at this step. In addition, during the post-APOBEC cleanup the beads tend to clump more easily. Do not over-dry the beads before DNA elution as this can result in difficulties in resuspending the beads, and potentially reduced DNA recovery.