HomeFAQsIs it possible to use total RNA input amounts outside the suggested range for the NEBNext UltraExpress® RNA library Kit?
FAQ: Is it possible to use total RNA input amounts outside the suggested range for the NEBNext UltraExpress® RNA library Kit?
Yes. However, it is possible that doing so may result in higher adaptor dimers or lower yields. Optimization of adaptor dilutions and PCR cycles should be performed experimentally by the user and will be affected by sample quality. We suggest using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7760/E7765) for total RNA inputs outside the ranges recommended for the NEBNext UltraExpress RNA Library Prep Kit.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.