HomeFAQsCan I perform poly(A) mRNA isolation or rRNA depletion and then freeze my samples at -80°C before beginning library preparation with the NEBNext UltraExpress® RNA Library Prep Kit?
FAQ: Can I perform poly(A) mRNA isolation or rRNA depletion and then freeze my samples at -80°C before beginning library preparation with the NEBNext UltraExpress® RNA Library Prep Kit?
Yes, however, some modifications will be required. If you plan to freeze your mRNA- or rRNA-depleted RNA before library prep, elution of your samples in 4 µl is recommended. 2.5µl of this eluate will then be transferred into a fresh tube and combined with an equivalent volume (2.5 µl) of NEBNext UltraExpress RNA Fragmentation Mix., for a total volume of 5 µl. Slightly greater sample loss is expected using this method compared to the standard protocol. However, adaptor input and cycling recommendations will remain the same.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
