If working with already fragmented, low integrity and/or FFPE DNA upstream of EM-seq library preparation (Section 3 of NEBNext UltraShear manual), for Step 3.1.6. we suggest fragmenting with NEBNext UltraShear for less time (5-15 minutes at 37˚C; optimizations may be needed). Additionally, we suggest performing 0.9X sample purification bead cleanups after Deamination of Cytosines (Step 3.9.2.) and after PCR Amplification (Step 3.11.3). Please note that we do not recommend a stringent sample purification bead cleanup (e.g., 0.65X) for already fragmented, low integrity and/or FFPE DNA samples.
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
