HomeFAQsWhy does digestion/editing efficiency differ between two different single guide RNAs?
FAQ: Why does digestion/editing efficiency differ between two different single guide RNAs?
Digestion efficiency may be influenced by single guide RNA design. Verify the sequence and design of the single guide RNA transcription template. It may also be influenced by single guide RNA quality. Verify the integrity of the single guide RNA by gel electrophoresis. Additionally, some DNA sites are more amenable to editing than others. Please test multiple target sites/single guide RNA sequences appropriate for the desired mutation.
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