Home FAQs What is the difference between EnGen® Seq1 Cas9, EnGen Sau Cas9 (NEB #M0654), and EnGen Spy Cas9 NLS (NEB #M0646)?

FAQ: What is the difference between EnGen® Seq1 Cas9, EnGen Sau Cas9 (NEB #M0654), and EnGen Spy Cas9 NLS (NEB #M0646)?

EnGen Seq1 Cas9, EnGen Sau Cas9, and EnGen Spy Cas9 NLS are Cas9 orthologs from Streptococcus equinus, Staphylococcus aureus, and Streptococcus pyogenes, respectively. They recognize the protospacer adjacent motifs (PAMs) of NAGA, NNGRRT, and NGG, respectively.