FAQ: What is the advantage of using phi29-XT DNA Polymerase instead of wild-type phi29?

Generally, phi29-XT DNA Polymerase, under optimal conditions, generates more product in less time than the wild-type enzyme. Additionally, phi29-XT is more sensitive than the wild-type phi29, making it ideal when the input DNA amount is extremely low (i.e., femtogram of circular DNA). These qualities make phi29-XT the ideal choice for most RCA applications.

Wild-type phi29 phi29-XT
Fast (3-12 kb/min)
Highly Processive
High Fidelity
Strand Displacement
Optimal Temperature 30°C 42°C
Yield ✓✓
Sensitivity ✓✓